Differences

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--- methods:sop:methazolamide [2023/07/14 13:48] – created ohardt
+++ methods:sop:methazolamide [2023/10/31 10:29] (current) – ohardt
@@ Line 2: / Line 2: @@
-  - Stock
+==== Preparing Stock Solution ====
-    - Molecular weight is 236.47 daltons
+  - Molecular weight of methazolamide is 236.47
-    - Stock concentration: 100 mM
+  - Stock concentration: 100 mM
-    - Take 50 mg methazolamide and add 2.1143 mL of DSMO
+  - Take 50 mg methazolamide and add 2.1143 mL of DSMO
-    - Aliquot into 20 µL aliquots and freeze in -80 C freezer
+  - Aliquot into 20 µL aliquots and freeze in -80 C freezer
+==== Preparing Infusion Solution ====
   - Dilute Stock to final concentration of 10 mM
-    - Take one 20 µL aliquot and add 180 µL DSMO
+  - Take one 20 µL aliquot and thaw it
-    - Briefly (3 sec) vortex
+  - Add 180 µL DSMO to aliquot
+  - Briefly (3 s) vortex