Forgetting & Memory Lab methods:sop http://hardtlab.com/ 2026-04-28T22:53:03+00:00 Forgetting & Memory Lab http://hardtlab.com/ http://hardtlab.com/lib/tpl/dokuwiki/images/favicon.ico text/html 2023-07-15T15:20:12+00:00 Anonymous (anonymous@undisclosed.example.com) methods:sop:animalnumbering http://hardtlab.com/doku.php?id=methods:sop:animalnumbering&rev=1689448812&do=diff Animal Numbering System Each animal will receive a unique ID with the format AA.cZZZ.XXX.YY * AA: Year animal joined the lab [2 digits] * ZZZ: Cage number for animal [3 digits] * XXX: Number of the animal in the current year [3 digits] * YY: Number of animal in the cohort in which it arrived [up to two digits] text/html 2023-07-15T15:20:11+00:00 Anonymous (anonymous@undisclosed.example.com) methods:sop:anisomycin http://hardtlab.com/doku.php?id=methods:sop:anisomycin&rev=1689448811&do=diff Anisomycin Recipe Ingredients * 1 bottle of saline, * Anisomycin, * 1N HCl * 1N NaOH Mixing Anisomycin (125 µg / µl) and Vehicle * Put container on a weight and re-zero (tare) * Weight 0.00625g of Anisomycin * Put Anisomycin in tube (or just weigh it in the tube) text/html 2023-07-15T15:20:11+00:00 Anonymous (anonymous@undisclosed.example.com) methods:sop:ap5 http://hardtlab.com/doku.php?id=methods:sop:ap5&rev=1689448811&do=diff AP5 Recipe Dissolve 2.5 µg AP5 powder per 1.0 µl of saline (in my experience this is a small concentration so you might need to dissolve 0.00125 g / 0.5 ml in order to get reliable weight measurements). This will be acidic so bring it up to 7.4 pH with a few µL of NaOH. text/html 2024-05-17T20:42:35+00:00 Anonymous (anonymous@undisclosed.example.com) methods:sop:experimentnumbering http://hardtlab.com/doku.php?id=methods:sop:experimentnumbering&rev=1715992955&do=diff Experiment Numbering and Shorthand All experiments will receive a unique ID that will be used in all documents for cross-reference. We also use a shorthand notation used together with the experiment ID in protocols and data files to describe the experimental procedure applied. text/html 2023-10-31T10:29:29+00:00 Anonymous (anonymous@undisclosed.example.com) methods:sop:methazolamide http://hardtlab.com/doku.php?id=methods:sop:methazolamide&rev=1698762569&do=diff Methazolamide Recipe Preparing Stock Solution * Molecular weight of methazolamide is 236.47 * Stock concentration: 100 mM * Take 50 mg methazolamide and add 2.1143 mL of DSMO * Aliquot into 20 µL aliquots and freeze in -80 C freezer Preparing Infusion Solution text/html 2023-07-15T15:20:11+00:00 Anonymous (anonymous@undisclosed.example.com) methods:sop:nor http://hardtlab.com/doku.php?id=methods:sop:nor&rev=1689448811&do=diff [SOP] Basic Object Recognition Protocol Apparatus * Open field * Dimensions: 600 mm X 600 mm X 600 mm * Walls: Laminated MDF (black or white) * Cue Cards: laminated black white pattern on North wall * Floor: laminated MDF (white), covered with approximately 5 cm of bedding used in the home cage. text/html 2023-07-15T15:20:12+00:00 Anonymous (anonymous@undisclosed.example.com) methods:sop:zipglua23y http://hardtlab.com/doku.php?id=methods:sop:zipglua23y&rev=1689448812&do=diff ZIP and GluA2-3Y Recipe TRIS-saline, pH 7.00 solution Add 1 mL of Tris 1 M (pH 7.00) to 9 mL sterile saline. Mix well. ZIP 10 nmol/µl * Add 104 µl of Tris-saline to a bottle containing 2 mg of ZIP (ZIP is in the -20 freezer in bottles containing 2 mg of lyophilized powder).